中国临床药学杂志

目的建立用于测定淫羊藿中淫羊藿苷含量的反相高效液相色谱法。方法采用ZORBAX Ecilpse XDB-C₁₈(150 mm×4.6 mm,5μm)色谱柱;以水-乙腈(74:26,V/V)为流动相;检测波长270 nm;流速1.0 mL·min^-1,柱温25℃。结果淫羊藿苷质量浓度在5～50 mg·L^-1内与其峰面积之间线性关系良好(r=0.999 9),平均加样回收率为100.32%,RSD为0.44%。结论本方法操作简便、快速,结果准确,可用于淫羊藿中淫羊藿苷的含量测定。

AIM To establish a reversed-phase high performance liquid chromatography method for the determination of icariin in Herba Epimedium.METHODS The analysis was performed on ZORBAX Ecilpse XDB-C₁₈(150 mm×4.6mm,5μm) column.The mobile phase consisted of water:acetonitrile(74:26,V/V) at a flow rate of 1.0 mL·min^-1 and the detection wavelength was 270 nm.The solution concentrations were detected by HPLC with the column temperature of 25℃.RESULTS Under the conditions described,the calibration curve of icariin was linear within the concentration range of 5-50mg·L^-1(r=0.9999,n=5).The average recovery was 100.32%.The mean relative standard deviation(RSD) was 0.44%.CONCLUSION The method is simple,accurate and suitable for the determination of icariin in Herba Epimedium.