中国临床药学杂志

目的建立一种用反相高效液相色谱法同时测定人血清中苯巴比妥、苯妥英钠和卡马西平的浓度。方法以艾司唑仑为内标物,血样经氯仿-乙酸乙酯提取后,氮气吹干,用甲醇复溶10μL进样,以Agilent Eclipse XDB-C18柱(150 mm×4.6 mm,5μm)为分析柱,甲醇-水(51∶49)为流动相,检测波长238 nm,流速1.0 mL·min^-1。结果苯巴比妥、苯妥英钠、卡马西平线性范围分别为2~80 mg·L^-1(r=0.999 2)、1.006~40.24 mg·L^-1(r=0.999 9)和0.5~20 mg·L^-1(r=0.999 2);相对回收率分别为101.10%、102.16%和100.84%,提取回收率均≥95%;日内、日间RSD均<6%。结论本次建立的HPLC法具有操作简便、快速,结果准确等优点,适于临床快速检测血清中苯巴比妥、苯妥英钠和卡马西平浓度。

AIM To establish a simple method for the simultaneous determination of phenobarbital,phenytoin and carbamazepine in human serum by reverse phase high performance liquid chromatography(RP-HPLC).METHODS Estazolam was used as internal standard.The samples were evaporated to dryness under N₂ stream after extracted by acetic ether and chloroform,then they were dissolved by methanol and analysized by HPLC with the Agilent Eclipse XDB-C₁₈column(150 mm×4.6 mm,5 μm).The mobile phase was methanol-water(51∶49),the detection wavelenth was 238 nm and the velocity was 1.0 mL·min^-1.RESULTS The standard curve of phenobarbital,phenytoin and carbamazepine was linear in the concentration range of 2-80 mg·L^-1,1.006-40.24 mg·L^-1 and 0.5-20 mg·L^-1 respectively.The relative recovery rates were 101.10%,102.16% and 100.84%,respectively.The extraction recovery rates were more than 95%.The RSDs of intra-day and inter-day were less than 6%.CONCLUSION The HPLC method is simple,rapid and accurate,and it is suitable for rapid drug monitoring of phenobarbital,phenytoin and carbamazepine.