中国临床药学杂志

目的:建立人参皂苷Rb1的HPLC测定方法,考察人参皂苷Rb1在胃液和肠液pH值下的稳定性。方法:人参皂苷Pb1测定以SHIMADZU-ODS(250 mm×4.6 mm,5μm)为固定相,乙腈-水(35:65,V/V)为流动相,紫外检测波长203 nm。分别考察人参皂苷Rb1在胃液、肠液pH值下的稳定性,同时采用液质联用技术分析人参皂苷Rb1在酸性条件下的降解产物。结果:人参皂苷Rb1进样量在60-600 mg·L-1范围内线性关系良好,r=0.999 9(n=6)。低、中、高浓度下回收率分别为99.25％,100.01％,100.39％,日内、日间RSD均<2％(n=5)。人参皂苷Rb1在肠液pH值下相对稳定,在胃液pH值下迅速降解,降解产物可能为人参皂苷Rb1失去1个或2个糖的产物。结论:本实验分析方法灵敏、准确、重现性好;人参皂苷Rb1在胃液pH值下不稳定。

AIM: To develop a method for the determination of ginsenoside Rb1 by HPLC. Stability of ginsenoside Rb1 and its decomposed products in gastric pH were also investigated. METHODS: Ginsenoside Rb1 was determined by a SHIMADZU-ODS column with mixture of acetonitril-water (35 : 65, V/V) as mobile phase at 203 nm. In addition, the stabilities of ginsenoside Rb1 were studied in gastric and intestinal pH. The decomposed products in gastric pH were analyzed by LC/MS. RESULTS: The linear range of Rb1 was 60-600 mg·L-1 with a correlation coefficient of 0.999 9 (n = 6). The average recoveries for Rb1 were 99.25% , 100.01% and 100.39% for 3 concentration, respectively. The within-day and the between-day RSD was lower than 2% (n = 5) . Ginsenoside Rbi in intestinal pH was stable, but in gastric pH it was decomposed rapidly. The decomposition products were the remained substances of Rb1 without one or two glucoses. CONCLUSION: The HPLC determination method of Rb1 is sensitive and accurate. Ginsenoside Rb1 in gastric pH is instable.