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目的了解广东珠海地区幽门螺杆菌(Hp)对常用抗菌药物的耐药情况,为临床规范化根除Hp提供理论依据。方法①内镜下于胃窦钳取1块黏膜进行快速尿素酶试验,阳性者再钳取1块黏膜行细菌培养。②Hp培养及鉴定:采用固体培养基分离培养及液体培养基扩增Hp菌株,经动力试验,革兰染色,生化试验包括尿素酶、过氧化氢酶、氧化酶试验鉴定为Hp菌株。③抗生素药敏试验:采用折点敏感试验方法测定Hp对5种不同抗菌药物的耐药情况。结果 Hp培养阳性率为30.4%(95/312);广东珠海地区Hp对5种抗菌药物甲硝唑、呋喃唑酮、阿莫西林、克拉霉素、左氧氟沙星的耐药率分别为88.4%(84/95)、61.1%(58/95)、47.4%(45/95)、42.1%(40/95)、21.1%(20/95)。Hp耐药率在性别、年龄、疾病类型间的差异无统计学意义(P>0.05)。结论 5种抗菌药物耐药率均较高,其中甲硝唑耐药率最高,左氧氟沙星耐药率最低。
Abstract：AIM To study the resistance of Helicobacter pylori(Hp) to commonly used antibacterial drugs in Zhuhai area to help instruct medication for Hp eradication.METHODS ①Two pieces of gastric antral mucosa were obtained,one for rapid urease enzyme test and the other for Hp culture if rapid urease enzyme test was positive.Hp was separated on agar base and cultured in fluid nutrient medium.②Hp colony was verified through gram staining,motility test and biochemical reactions including catalase,oxidase and urease.③The drug resistance of Hp to 5 different antibacterial drugs was tested by breakpoint susceptibility testing.RESULTS The positive rate of culture was 30.4%.The resistance rates against metronidazole,furazolidone,amoxicillin,clarithromycin and levofloxacin were 88.4%,61.I%, 47.4%,42.1%and 21.1%,respectively.There were no significant statistical differences of Hp resistance between disease state,sex or among different age groups(P>O.05).CONCLUSION This study showes that antibiotic resistance rates of Hp in Zhuhai are generally high,within which metronidazole is the highest and levofloxacin is the lowest.
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基本信息:
DOI：10.19577/j.cnki.issn10074406.2013.04.009
中图分类号:R446.5
引用信息:
[1]卫金歧,农高惠,陈美竹,等.广东珠海地区幽门螺杆菌对常用抗菌药物的体外耐药情况[J].中国临床药学杂志,2013,22(04):234-237.DOI:10.19577/j.cnki.issn10074406.2013.04.009.
基金信息:
广东省珠海市科技工贸和信息化局项目(编号2011B040102006)
2013-07-25
2013-07-25