中国临床药学杂志

目的建立清胃合剂盐酸小檗碱和黄芩苷含量的测定方法。方法采用反相HPLC法同时测定清胃合剂中盐酸小檗碱和黄芩苷的含量。流动相为乙腈-0.1 mol·L-1磷酸二氢钾(25∶75),检测波长265 nm。结果盐酸小檗碱质量浓度为14.5~232 mg·L-1,黄芩苷质量浓度为35.5~568 mg·L-1时线性关系良好,平均回收率分别为103.15%和100.27%。结论本法测定结果准确,适用于该制剂的含量测定。

AIM To establish a method for determing berberine hydrochloride and Baicalin in Qinwei Liquor. METHODS An HPLC method was developed to determine berberine hydrochloride and Baicalin in Qinwei Liquor. The mobile phase consisted of acetonitrile-0. 1mol·L-1potassium dihydrogen phosphate( 25∶ 75),and the detecting wavelength was 265 nm. RESULTS Good linearity within the linear range from 14. 5 to 232 mg·L-1for berberine hydrochloride,and from 35. 5 to 568 mg·L-1for baicalin was obtained. The average recovery was103. 15% and 100. 27% for berberine hydrochloride and Baicalin,respectively. CONCLUSION The method is accurate,and suitable for the determination of the preparation.