| 44 | 0 | 22 |
| 下载次数 | 被引频次 | 阅读次数 |
本文报道采用SE-54石英毛细管色谱柱及电子捕获检测器(63Ni-ECD)毛细管气相色谱法(CGC)测定了大鼠血浆中β-苯基乳酸(PLA)的浓度,线性范围1.6～9.6ng,相关系数r=0.9997,检测限为0.5ng;采血量为0.1ml,并用醋酸乙酯对大鼠血浆中PLA进行提取,方法灵敏,回收率高,平均回收率为99.04％。日内与日间平均变异系CV分别为5.1272％,5.9974％。此方法可用于临床药代动力学研究。
Abstract：The determination of β-Phenylactic acid (PLA) in rat's plasma by capillary chromatography is described. Quantitation was effected by 63 Ni-electron capture detector after separation with a SE-54 quartz capillary chromatographic column. Claibration curves of the instrumental responses against drug concentrations were linear within the range 1. 6-9. 6ng. The limit of detection of PLA wae considered to be 0. 5ng. PLA was extracted from plasma with ethyl acetate (rat's plasma 0.1ml). The recovery from plasma was higher and the mean valune was 99. 04%. Their coefficents of variation (CV) of within day and between day were 5. 1272%, and 5. 9974% respectively.
1 Clyde m. williams. Determination of β-phenyllactic Acid by Gas Chromatography. Analylical BIochemistry 1963;6: 468～471
2 E. R. Blakley Gas Chromatography of Phnolic Acids. Analylical BIochemistry 1965;6: 350～354．
3 Nonrman E. Hoffnan and karen m. gooding, gas Chromatography of Some Urinay Acid Metabolites Related to phenylketonuria Analylical Biochemistry 1969;31: 471～479
4 杨丽莉,等.抗心律失常新药关附甲素血浓度测定的方法研究 华东地区第三次药物分析学术交流会
5 衍生气相色谱及应用 湖南大学出版社 1978
6 Daniel R. knapp. Handbook of Analytical Derivatiz ation Reactions. New York Chichester Brisbane Toronto
基本信息:
DOI：10.19577/j.cnki.issn10074406.1994.02.001
中图分类号:R96
引用信息:
[1]莫谷强,张晓敏,程务本,李端,杨香媛.毛细管气相色谱法测定大鼠血浆中β-苯基乳酸浓度[J].临床药学,1994(02):1-4.DOI:10.19577/j.cnki.issn10074406.1994.02.001.
1994-06-30
1994-06-30