中国临床药学杂志

目的建立参七胶囊的制备工艺及其质量控制的薄层色谱(TLC)法。方法将三七、人参、丹参用适宜的方法提取并制成胶囊。采用TLC法,对参七胶囊进行定性鉴别。以三氯甲烷-甲醇-水(65∶35∶10)为展开剂,10%硫酸乙醇溶液为显色剂,检测人参皂苷Rb1、Rg1,三七皂苷R1;以甲苯-醋酸乙酯-甲酸(8∶5∶0.8)为展开剂,2%三氯化铁溶液与1%铁氰化钾溶液(1∶1)为显色剂,检测原儿茶醛。结果用TLC法分别检出参七胶囊中含人参皂苷Rb1、Rg1,三七皂苷R1及原儿茶醛。结论本品制备工艺可靠,质量可控。

AIM To establish the manufacturing technology of Shenqi capsules and its quality control of thin-layer chromatography(TLC) method. METHODS The Sanchi,Ginseng and Dan-shen root were refined and made into capsules. Using TLC method,the Shenqi capsules charaeteristics were tested. Using chloroform-methanol-water (65∶35∶10) as the developing system, 10% of ethand sulfate as developer,qinsenoside Rb₁ and Rg₁ and notoginsenosideR₁ were tested; using toluene-ethylacetate-formc acid(8∶5∶0.8) as the developing system, 2% of ferric chloride liquid together with 1% of potassium ferrocy as developer,protocatechual dehyde was tested. RESULTS Qinsenoside Rb₁ and Rg₁ and notoginsenosideR₁ and protocatechual dehyde were tested in the Shenqi capsules by way of TLC. CONCLUSION The manufacturing technology of this product is reliable and its quality can be controlled.