中国临床药学杂志

目的 建立菟丝育长口服液的指纹图谱，为制剂的质量控制提供参考。方法 采用HPLC法，色谱柱为Aglient Zorbax SB-C₁₈色谱柱(250 mm×4.6 mm,5μm)；流动相A为乙腈，流动相B为0.1%磷酸水溶液，梯度洗脱，流速为1.0 m L·min^-1，柱温30℃，检测波长254nm。结果 10批菟丝育长口服液的相似度均在0.95以上，得到了32个共有峰，指认了莫诺苷、绿原酸、去甲异波尔定、马钱苷、松果菊苷、淫羊藿苷、肉桂酸、甘草酸8种成分。结论 该研究建立的菟丝育长口服液的指纹图谱，具有简便可靠，重复性好等特点，可为制剂的质量控制提供参考。

AIM To establish the fingerprints of Tusiyuzhang oral liquid and provide reference basis for the quality control of the preparation. METHODS The chromatographic column was Aglient Zorbax SB-C₁₈ column(250 mm ×4.6 mm, 5 μm) by HPLC with gradient eluting. The mobile phase A was acetonitrile and the mobile phase B was 0.1% phosphoric acid aqueous solution; the column temperature was 30 ℃; the detection wavelength was 254 nm. RESULTS The similarity of 10 batches of Tusiyuzhang oral liquid was more than 0.95, 32 common peaks were obtained, and 8components were identified: morroniside, chlorogenic acid, norisoboldine, loganin, echinacoside, icariin, cinnamic acid,and glycyrrhizic acid. CONCLUSION The fingerprints of Tusiyuzhang oral liquid established in this study are simple,reliable and reproducible. It can provide reference for the quality control of the preparation.