中国临床药学杂志

目的研究不同浓度培哚普利对小鼠胚胎成骨细胞前体细胞MC3T3-E1增殖、分化的影响,从而探讨培哚普利对成骨细胞的作用。方法体外培养MC3T3-E1细胞,构建细胞实验模型;采用CCK-8法检测不同浓度(0、1×10^-3、1×10^-4、1×10^-5、1×10^-6 mol·L^-1)培哚普利干预下MC3T3-E1细胞的增殖变化;分别用不同浓度培哚普利干预MC3T3-E1细胞3、5及7 d,用碱性磷酸酶(ALP)活性试剂盒检测其活性。结果培养的MC3T3-E1细胞生长活跃,状态良好。细胞经培养48 h后,1×10^-5 mol·L^-1培哚普利产生促细胞增殖作用(P<0.05),1×10^-6 mol·L^-1培哚普利促细胞增殖作用明显(P<0.01);细胞经不同浓度培哚普利作用3、5及7 d后,与溶媒对照组相比,培哚普利各组细胞ALP活性之间差异均无统计学意义(P>0.05)。结论一定时间内,一定浓度的培哚普利对体外培养的MC3T3-E1细胞具有促增殖作用,但对其早期分化没有影响。

AIM To explore the role of perindopril on osteoblasts by studying the influences of different concentrations of perindopril on proliferation and differentiation of mouse embryonic osteoblast precursor MC3 T3-E1 cells. METHODS Proliferation of MC3 T3-E1 cells was detected by CCK-8 assay with different concentrations(0,1×10^-3,1×10^-4,1×10^-5 and 1×10^-6 mol·L^-1) of perindopril. The MC3 T3-E1 cells were cultured with different concentrations of perindopril for 3, 5 and 7 d, then alkaline phosphatase(ALP) activity was detected with the ALP activity kit. RESULTS MC3 T3-E1 cells grew actively, in good condition. After culturing for 48 h, cells cultured with 1×10^-5 mol·L^-1 perindopril had pro-proliferation effect(P<0.05), 1×10^-6 mol·L^-1 perindopril had obvious pro-proliferation effect(P<0.01).After 3, 5 and 7 d of perindopril treatment at different concentrations,there was no statistically significant difference in the alpine activity between the perindopril groups and the solvent control group(P>0.05). CONCLUSION Within a certain period of time and a certain concentration in vitro, perindopril can promote the proliferation of MC3 T3-E1 cells, but has no effect on the early differentiation.