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目的 :进行板蓝根低极性流分及其亚流分的分离 ,并研究其免疫活性。方法 :用快速低压干柱柱层析从板蓝根中分离出一个低极性流分及其一组亚流分 ;用化学发光法检测其对人多形核细胞化学发光的影响。结果 :板蓝根低极性流分具有双向免疫活性 ,在低浓度时具有激活作用 ,在高浓度时具有抑制作用 ;而亚流分的免疫活性并未因其纯度的提高而相应提高。结论 :板蓝根低极性流分及其亚流分含有抗炎成分。
Abstract：AIM: To make isolation of a low polar fraction and its subfractions from Banlangen, dried root of Isatis tinctoria L , and study their immunocompetence. METHODS: A low polar fraction and its subfractions were isolated from Banlangen with dry column flash chromatography. It was tested that the effect of those fractions on chemiluminescence reaction of human polymorphonuclear cells. RESULTS: A two way immunocompetence was found from those fractions. The effect was activation while the concentrations of those fractions were low, and it was inhibition while their concentrations were high. Immunocompetence of the subfractions did not increase while their degree of purity increased. CONCLUSION: There are some anti inflamation components in a low polar fraction and its subfractions from ‘Banlangen’.
[1] Honda G,Tosirisuk V,Tabata M.Isolation of an antidermato-phytic,tryptanthrin,from indigo plants,polygonum tinctoriumand isatis tinctoria.Planta-Medica,1980 ,3 8( 5 ) :2 75
[2 ] Huang Qiaoshu,Yoshihira K,Natori S. Isolation of 2 -hydroxy-3 -butenyl thiocyanate,epigoitrin,and adenosine from banlan-gen,Isatis indigotica root. Planta-Medica,1981,42 ( 6) :3 0 8
[3 ]游松 ,姚新生 ,陈英杰 ,等 .中药板蓝根中活血有效成分的研究 .中药通报 ,1988,13 ( 2 ) :3 1
[4]李玲 ,杨根金 ,董同义 ,等 .菘蓝化学成分研究 .中草药 ,1996,2 7( 7) :3 89
[5 ]刘云海 .板蓝根抗内毒素作用研究 .中国药科大学学报 ,1995 ,2 6( 5 ) :2 97
[6]胡天喜 ,陈杞 ,陈克明 ,等 .发光分析与医学 .上海 :华东师范大学出版社 ,1990 ,89
[7] Andersen L,Nielsen BS,Norgaard A,et al.Measurement ofthe interation between H elicobacter pylori and phagocytic cellsinduction of the oxidative burst,phagocytosis and bactericidalactivity. H elicobacter pylori:techniques for clinical diagnosisand basic research.Adrian L,etal. ( eds) WB Saunders Com-pany LTD,London,1996:2 45
基本信息:
DOI：10.19577/j.cnki.issn10074406.2001.01.011
中图分类号:R285
引用信息:
[1]秦箐,贺海平,SorenBroggerChristensen,HasseBondeRasmussen,ArsalanKharazmi,陈鸣.板蓝根低极性流分的分离及其免疫活性[J].中国临床药学杂志,2001(01):29-31.DOI:10.19577/j.cnki.issn10074406.2001.01.011.
基金信息:
中国一丹麦科技合作协议 Danida资助项目!(项目编号 AM9∶ 3 )