中药枳实对功能性消化不良大鼠胃排空的影响Effect of immature bitter orange on gastric emptying in rats with functional dyspepsia
朱金照,张志坚,张捷
摘要(Abstract)：
目的研究枳实对功能性消化不良大鼠胃排空的影响及其可能机制。方法健康成年Wistar大鼠30只,随机分为正常对照组、模型组和模型+枳实组。测定各组大鼠胃排空速率,并对大鼠胃窦组织进行P物质、胃动素及血管活性肠肽免疫组化染色。结果模型组大鼠胃排空速率明显低于正常对照组(P<0.01)。应用枳实水提取液后大鼠胃排空速率增加,与正常对照组无明显差异(P>0.05)。模型组大鼠胃窦组织P物质、胃动素明显低于正常对照组(P<0.01或P<0.05),血管活性肠肽明显高于正常对照组(P<0.05)。应用枳实水提取液后大鼠胃窦组织P物质、胃动素明显增加,血管活性肠肽明显减少,与正常对照组无明显差异(P>0.05)。结论枳实可改善功能性消化不良大鼠的胃排空,其机制可能与胃窦组织P物质、胃动素的分布增加及血管活性肠肽的减少有关。
关键词(KeyWords)： 枳实;P物质;血管活性肠肽;胃动素
基金项目(Foundation):
作者(Author): 朱金照,张志坚,张捷
DOI: 10.19577/j.cnki.issn10074406.2005.05.009
参考文献(References)：
- [1]朱金照,冷恩仁,陈东风,等.15味中药促胃肠动力作用的筛选研究[J].第三军医大学学报,2000,22(5):436.
- [2]朱金照,冷恩仁,张捷,等.大腹皮对大鼠胃电节律紊乱的影响及机制[J].解放军医学杂志,2002,27(1):39.
- [3]李岩,陈苏宁,陈少夫,等.柴胡枳实合剂对健康人胃排空,小肠推进功能及血中胃动素,胃泌素水平的影响[J].中国中西医结合杂志,1996,16(6):622.
- [4]邹燕琴,赖天松,李政木,等.枳实消痞丸对功能性消化不良患者胃动力学及胃肠激素的影响[J].广州中医药大学学报,1999,16(4):305.
- [5]王翠芬,杨泛治,魏义全,等.枳实对大鼠胃肠肌电活动影响的初步研究[J].东南大学学报(医学版),2001,20(3):153.
- [6]Maake C,Kloas W,Szendefim,et al.Neurohormonal peptides,sero-tonin and nitric oxide synthase in the enteric nervous system and en-docrine cells of the gastrointestinal tract of neotenic and thyroid hormone-treated axolotls[J].Cell Tissue Res,1999,297(1):90.
- [7]Lordal M,Theodorsson E,Hellstrom PM.Tachykinms influence interdi-gestive rhythm and contractive strength of human small intestine[J].DigDis Sci,1997,42(9):1940.
- [8]Wheatley IM,Hutson JM,Chow CW,et al.Slow-transit constipation inchildhood[J].J Pediatr Surg,1999,34(5):829.
- [9]Sasaki W,Yoshihara T.The effect of motilin on the regulation mecha-nism of intestinal motility in conscious horses[J].J Vet Med Sci,1999,61(2):167.
- [10]Ljung T,Hellstrom PM.Vasoactive intestive peptide suppress migrat-ing myoelectric complex of rat small intestinal indepent of nitric oxide[J].Acta Physiol Scand,1999,165(2):225.
- [11]Li LS,Qu RY,Wang W,et al.Significance of changes of gastroin-testinal peptides in blood and ileum of experimental spleen deficiencyrats[J].World J Gastroenterol,2003,9(3):553.
- [12]Sandgren,K,Lin Z,Svenningsen AF,et al.Vasoactive intestinalpeptide and nitric oxide promote survival of adult rat myenteric neuronsin culture[J].J Neurosci Res,2003,72(5):595.