自动柱切换高效液相色谱法同时测定血浆中的SMZ和TMPDETERMINATION OF SMZ AND TMP SIMULTANEOUSLY USING AUTOMATED COLUMN-SWITCHING HPLC TECHNIQUE
王卓,高申,刘荔荔,吴玉田,谈家善,毛伯义
摘要(Abstract)：
应用自制自动柱切换装置建立了同时测定SMZ及TMP血药浓度的HPLC方法.采用μBondapak C 18(50mm×4.6mm.37～50μm)为预处理柱,水为预处理流动相.分析柱为Hypersil BDS C18(150mm×4.6mm,5μm)柱,分析流动相为甲醇:磷酸缓冲液=35:65.检测波长230nm.SMZ和TMP分别在2.5～80μg/ml及0.125～4μg/ml范围内线性良好(r_1=0.999 7,r_2=0.9998).平均方法回收率分别为98.59%及95.71%;日内精密度分别为2.11%及2.67%,日间精密度分别为2.51%及2.89%;两者的最低血浆检测浓度可达SMZO.1μg/ml,TMPO.02μg/ml.方法迅速、简便、灵敏.
关键词(KeyWords)： 磺胺甲基异(口恶)唑;甲氧苄胺嘧啶;血药浓度;自动柱切换;高效液相色谱法
基金项目(Foundation):
作者(Author): 王卓,高申,刘荔荔,吴玉田,谈家善,毛伯义
DOI: 10.19577/j.cnki.issn10074406.1997.03.009
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