MTS比色法检测非小细胞肺癌细胞体外对常用化疗药物的敏感性Study on sensitivity of cancer cells of non-small cell lung cancer to commonlyused chemotherapeutic drugs by MTS assay
顾梅;陈娟;汪小乐;程国华;
摘要(Abstract)：
目的采用MTS比色法检测非小细胞肺癌(NSCLC)患者癌性胸水中肿瘤细胞对常用化疗药物的敏感性。方法采用密度梯度离心法提取19例NSCLC患者癌性胸水中的肿瘤细胞,应用MTS比色法检测其对顺铂(DDP)、多西他赛(DOC)、长春瑞滨(NVB)、氟尿嘧啶(5-FU)、博来霉素(BLM)、奈达铂(NDP)、吉西他滨(GEM)、培美曲塞(MTA)等8种化疗药物的敏感性,以及这8种化疗药物对NSCLC胸水细胞的抑制作用。结果 19例NSCLC患者胸水标本中肿瘤细胞均成功提取。各化疗药物对NSCLC抑制作用之间差异均有统计学意义(P<0.001),从高到低依次为:NDP>DDP>GEM>NVB>5-FU>MTA>DOC>BLM。随着药物浓度的增加,各化疗药物对肿瘤细胞的抑制作用逐渐增大,癌性胸水中肿瘤细胞对各化疗药物的敏感性增加。结论 MTS比色法作为一种简便、快速、安全、灵敏性高的体外药敏试验方法,可用于NSCLC患者的个体化治疗选择合适的化疗药物。
关键词(KeyWords)： MTS比色法;癌性胸水;化疗药物;敏感性
基金项目(Foundation):
作者(Authors): 顾梅;陈娟;汪小乐;程国华;
DOI: 10.19577/j.cnki.issn10074406.2014.03.005
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