中药循经敷贴对哮喘豚鼠肺组织Eotaxin mRNA和IL-5 mRNA表达的影响Effect of herbal penetration therapy on Eotaxin mRNA,IL-5 mRNA expression in lung tissue of guinea pigs with experimental allergic asthma
沈惠风;刘烨;王利民;
摘要(Abstract):
目的探讨中药循经敷贴治疗哮喘的作用机制。方法采用Hutson法豚鼠模型,治疗组隔日诱喘后用中药敷贴30min;对照组隔日诱喘后采用生理盐水浸润纱布敷贴,时间同治疗组。记录各组诱喘潜伏期时间。2周后处死豚鼠,取肺组织,用半定量RT-PCR法比较Eotaxin mRNA、IL-5 mRNA的表达。结果中药循经敷贴能显著延长豚鼠诱喘潜伏期,下调哮喘豚鼠肺组织Eotaxin mRNA、IL-5 mRNA的表达。结论抑制变异性炎症可能是中药循经敷贴治疗哮喘的重要机制之一。
关键词(KeyWords): 哮喘;中药;Eotaxin mRNA;IL-5 mRNA
基金项目(Foundation): 上海市科学技术发展基金资助(编号024119084)
作者(Authors): 沈惠风;刘烨;王利民;
DOI: 10.19577/j.cnki.issn10074406.2010.04.007
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