硫辛酸抗神经细胞老化的蛋白质组学分析Proteomic analysis of anti-aging effect of lipoic acid in cultured neurons
张毅;苗玲;苏敏;蔡琰;
摘要(Abstract)：
目的研究硫辛酸在神经细胞老化过程中对细胞保护作用的蛋白质分子基础。方法采用无血清培养神经母细胞瘤细胞方法建立实验性神经细胞老化模型。设立硫辛酸(LA)作用组和对照组,运用蛋白质组学技术平台,采用双向电泳结合基质辅助激光电离-飞行时间质谱技术分析,并鉴定表达受到硫辛酸调控的蛋白。结果经蛋白质组学技术分析,发现硫辛酸能阻止19个蛋白在老化过程中表达改变,成功鉴定出其中5个蛋白,其中STMN1、TPM4、SERB和PARK7在老化过程中表达下降,DLDH在老化过程中表达上升。结论硫辛酸抗老化保护神经细胞的作用机制涉及到对STMN1、TPM4、SERB、PARK7和DLDH等蛋白的调控。
关键词(KeyWords)： 硫辛酸;神经细胞老化;蛋白质组学
基金项目(Foundation):
作者(Authors): 张毅;苗玲;苏敏;蔡琰;
DOI: 10.19577/j.cnki.issn10074406.2010.06.008
参考文献(References)：
- [1]蔡琰,沈金坤,陆荣华.神经细胞老化实验研究模型:无血清条件下培养小鼠神经母细胞瘤细胞[J].实验生物学报,1985,18(6): 453.
- [2]张毅,苗玲,苏敏,等.硫辛酸在神经细胞老化过程中的细胞保护作用研究[J].中国老年学杂志,2009,29(13):1611.
- [3]Hofman A,de Jong P,van Duijn CM,et al.Epidemiology of neurological diseases in elderly people:what did we learn from the rotterdam study?[J].Lancet Neurol,2006,5(6):545.
- [4]Reynolds A,Laurie C,Mosley RL,et al.Oxidative stress and the pathogenesis of neurodegenerative disorders[J].Int Rev Neurobiol,2007,82: 297.
- [5]Trushina E,McMurray CT.Oxidative stress and mitochondrial dysfunction in neurodegenerative diseases[J].Neuroscience,2007,145(4): 1233.
- [6]Holmquist L,Stuchbury C,Berbaum K,et al.Lipoic acid as a novel treatment for Alzheimer's disease and related dementias[J].Pharmacol Ther,2007,113(1):154.
- [7]张毅,苗玲,苏敏,等.硫辛酸对神经细胞老化过程中细胞凋亡的抑制作用[J].上海交通大学学报(医学版),2008,28(11):1416.
- [8]Abouhamed M,Reichenberg S,Robenek H et al.Tropomyosin 4 expression is enhanced in dedifferentiating smooth muscle cells in vitro and during atherogenesis.Eur J Cell Biol,2003,82(9):473.
- [9]Nakano I,Dougherty JD.,Kim K,et al.Phosphoserine phosphatase is expressed in the neural stem cell niche and regulates neural stem and progenitor cell proliferation.Stem Cells,2007,25(8):1975.
- [10]Belmont ID,Mitchison TJ.Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules.Cell, 1996,84(4):623.