硫辛酸抗神经细胞老化的蛋白质组学分析Proteomic analysis of anti-aging effect of lipoic acid in cultured neurons
张毅;苗玲;苏敏;蔡琰;
摘要(Abstract)：
目的研究硫辛酸在神经细胞老化过程中对细胞保护作用的蛋白质分子基础。方法采用无血清培养神经母细胞瘤细胞方法建立实验性神经细胞老化模型。设立硫辛酸(LA)作用组和对照组,运用蛋白质组学技术平台,采用双向电泳结合基质辅助激光电离-飞行时间质谱技术分析,并鉴定表达受到硫辛酸调控的蛋白。结果经蛋白质组学技术分析,发现硫辛酸能阻止19个蛋白在老化过程中表达改变,成功鉴定出其中5个蛋白,其中STMN1、TPM4、SERB和PARK7在老化过程中表达下降,DLDH在老化过程中表达上升。结论硫辛酸抗老化保护神经细胞的作用机制涉及到对STMN1、TPM4、SERB、PARK7和DLDH等蛋白的调控。
关键词(KeyWords)： 硫辛酸;神经细胞老化;蛋白质组学
基金项目(Foundation):
作者(Author): 张毅;苗玲;苏敏;蔡琰;
Email:
DOI: 10.19577/j.cnki.issn10074406.2010.06.008
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